human osteoblast growth medium  (Merck & Co)

Journal: BMC Oral Health

Article Title: The prohibitin ligand IN44 decreases Porphyromonas gingivalis mediated inflammation

doi: 10.1186/s12903-024-05209-2

Figure Lengend Snippet: - Effect of IN44 on metabolic activity and cell viability in periodontal cells. The impact of IN44 (10,50,100 µM) on the metabolic activity and cell viability of epithelial cells, fibroblasts, osteoblasts was measured by AlamarBlue assay ( A ) and Live/Dead assay ( B ) respectively after 24 h of exposure. Live cells in green, dead cells in red (* p < 0.01 compared to control group). One-way ANOVA and Dunnett post hoc

Article Snippet: Primary human osteoblasts (Promocell, Heidelberg, Germany) were cultured in Osteoblast Growth Medium with Osteoblast Growth Medium Supplement Mix (Promocell, Heidelberg, Germany).

Techniques: Activity Assay, Alamar Blue Assay, Live Dead Assay, Control

Journal: BMC Oral Health

Article Title: The prohibitin ligand IN44 decreases Porphyromonas gingivalis mediated inflammation

doi: 10.1186/s12903-024-05209-2

Figure Lengend Snippet: - Effect of IN44 on mitochondrial superoxide production in infected and treated epithelial cells after 45 min and 3 h. Mitochondrial superoxide production in live epithelial cells was detected with MitoSOX by fluorescence microscopy after 45 min–3 h of P. gingivalis infection (Pg) and treatment with IN44 (50 µM) (Pg IN44). Scale bars are 40 μm ( A ). Quantitative fluorescence intensity was analyzed by ECHOPro application ( B ) (* p < 0.05 compared to control, † p < 0.05 compared to infected cells). Scale bars are 170 μm for epithelial cells and 430 μm for fibroblasts and osteoblasts. One-way ANOVA and Dunn post hoc

Article Snippet: Primary human osteoblasts (Promocell, Heidelberg, Germany) were cultured in Osteoblast Growth Medium with Osteoblast Growth Medium Supplement Mix (Promocell, Heidelberg, Germany).

Techniques: Infection, Fluorescence, Microscopy, Control